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Anti-Xyloglucan (monoclonal, clone LM25)
Product no: AS18 4205-1ml
AS18 4205-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
260
€
Delivery:
3-6 business days
- Product Info
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Sub class: IgM Immunogen: Neoglycoprotein, Xyloglucan Host: Rat Clonality: Monoclonal Purity: Cell culture supernatant. Format: Liquid Quantity: 1 ml Storage: Store at +4°C (short term) and at -20°C (long term). Tested applications: ELISA (ELISA), Immuniflourescence (IF) Recommended dilution: 1:10 (ELISA, IF) - Reactivity
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Confirmed reactivity: Higher plants, ferns and mosses Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Material: Cassava leaf, epidermal layer
Fixation: aldehyde, embedded in HM20 resin, thin sectioned
Blocking: 4% milk in PBS
Primary antibody: Anti-xyloglucan, LM25 (AS18 4205-1ml)
Secondary antibody (Agrisera): Donkey anti-Rat IgG (H&L), DyLight® 594 conjugated, AS12 2090 (red)
Counterstained with Calcofluor White (blue). Super-resolution images were acquired on a ZEISS Elyra7 in Lattice Structured Illumination Microscopy mode.
Courtesy Dr. Kirk Czymmek, Donald Danforth Plant Science Center, USA - Additional Information
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Additional information: Contains 0,05% Sodium Azide - Background
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Background: The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Xyloglucan is a hemiceullose or polysaccharide that can be found in the primary cell wall. - Product Citations
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Selected references: Pedersen et a. (2012). Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem. 2012 Nov 6;287(47):39429-38.doi: 10.1074/jbc.M112.396598. - Protocols
- Immunofluorsecence as described in: Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.
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From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
260 €
AS18 4204-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
260 €