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Anti-TPS1 | Trehalose-6-phosphate synthase 1
AS12 2635 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana TPS1, UniProt: Q9SYM4, TAIR: AT1G78580
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 5000 (WB) Expected | apparent MW: 105.9 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Camelia sinensis, Vitis vinifera
Species of your interest not listed? Contact usNot reactive in: Cucumis sativus, Monocots - Application Examples
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Application example
Total protein from Arabidopsis thaliana wilde type (1,2), knowck out mutant (3) and overexpression line (4) were extracted with 50 mM HEPES/NaOH (pH 7.5), 1 mM EDTA, 1 mM DTT (+Protease Inhibitor cocktail) and denatured at 100°C for 5 min. Proteins were separated on 10 % SDS-PAGE and blotted overnight at 4°C to PVDF using wet tank transfer. Blots were blocked with 5 % low fat milk in 1xTBS-T for 1h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in 2 % low fat milk in 1xTBS for 1h at RT with agitation. The blot was washed as above and developed. Exposure time was 10 seconds.
Courtesy of Dr. Vasiliki Zacharaki, Umeå Plant Science Centre, Sweden
10 µg of total protein from Nicotiana benthamiana leaves expressing AtTPS1-GFP were separated on 10% SDS-PAGE using semi-dry transfer and blotted 1h to PVDF. Crude: non-purified material. Purified: immunoprecipitated with anti-GFP beads. Blots were blocked with 5% skimmed milk powder dissolved in TBS-T (0.1 % Tween 20) at 4°C ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 for 1h at room temperature (RT) with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 25 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was 30 seconds.
Courtesy of Dr. Takeo Sato, Hokkaido University, Japan - Additional Information
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Additional information (application): So far this antibody was not used on endogenous extracts from Arabidopsis thaliana - Background
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Background: TPS1 (Trehalose-6-phosphate synthase 1), EC=2.4.1.15 is an enzyme required for normal embryo development, vegetative growth and transition to flowering. It is involved in the regulation of glucose sensing. Expressed in seedlings, leaves, roots, stems, flowers and siliques. Up-regulated during seed development. Alternative names: alpha-trehalose-phosphate synthase [UDP-forming] 1.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection
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