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Anti-Tom 9,2 | Mitochondrial import receptor subunit TOM9,2
AS15 2902 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana
- Product Info
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Immunogen: Recombinant full length protein (coding region) of Tom9.2 Arabidopsis thaliana UniProt: Q9FNC9 TAIR: AT5G43970
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 10 | 9 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Hordeum vulgare, Musa sp., Oryza sativa, Phaseolus vulgaris, Physcomitrium patens, Ricinus communis, Solanum tuberosum, Triticum aestivum, Zea mays
Species of your interest not listed? Contact usNot reactive in: Ostreococcus tauri
- Additional Information
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Additional information (application): Antibody works on whole leaf extracts and isolated mitochondria; requires Tricine gels for sharp bands due to the small MW - Background
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Background: TOM9 (Mitochondrial import receptor subunit TOM9) is a central component of the receptor complex responsible for recognition and translocation of cytosolically synthesized mitochondrial preproteins.
Alternative names: Mitochondrial import receptor subunit TOM22 homolog 2, Translocase of outer membrane 22 kDa subunit homolog 2, Translocase of outer membrane 9 kDa subunit TOM9-2 - Product Citations
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Selected references: Kolodziejczak et al. (2018). m-AAA Complexes Are Not Crucial for the Survival of Arabidopsis Under Optimal Growth Conditions Despite Their Importance for Mitochondrial Translation. Plant Cell Physiol. 2018 May 1;59(5):1006-1016. doi: 10.1093/pcp/pcy041. - Protocols
- Reviews:
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Peter Ma | 2019-04-12This antibody works very well for our samples. The membrane proteins from the Arabidopsis leaves and roots are subjected to 15% SDS-PAGE and then detected with this antibody (1:5000). The results shows that there is a very clearly single band around 10kD.Malgorzata Heidorn-Czarna | 2017-10-17The antibody yields a very strong and clean signal on western blots with isolated mitochondrial proteins from Arabidopsis seedlings and leaves. It also works well with the total protein extract obtained from dry and germinating Arabidopsis seeds. We use 10-20 µg of mitochondrial proteins or 30-40 µg of total seed protein extract with 1:1000 dilution of the antibody followed by standard ECL detection. Single band of ca. 10 kDa is detected on the membrane.
Accessories
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