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Anti-Tlp18,3 | Thylakoid lumen 18,3 kDa protein
AS08 369 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Z.mays
- Product Info
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Immunogen: synthetic peptides amino acids 227-241 and amino acids 242-256 from Arabidopsis thaliana Tlp18.3 protein sequence TAIR: At1g54780)
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 22 | 18.5 kDa (A. thaliana)
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Oryza sativa, Ricinus communis, Zea mays
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
4, 2, and 1μg chl/lane (from left to right) of chloroplast prepared from Arabidopsis thaliana were separated on a 15% gel with 6M urea and blotted to PVDF. Filters were blocked (1h) with 5% milk powder and incubated with anti-Tlp18.3 antibody (1: 1000, 1h) followed by incubation with secondary HRP-coupled anti-rabbit antibody (1: 10 000, 1h). Signal was detected with chemiluminescence. - Additional Information
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Additional information (application): Application note: in a gel without urea Tlp18,3 will run at 18 kDa, while in a gel with urea it runs at 25 kDa - Background
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Background: UPF0603 protein At1g54780, chloroplastic is located in thylakoid lumen. The protein seem to be required for high light tolerance of PSII. Sirpiö et al. (2007) TLP18.3, a novel thylakoid lumen protein regulating photosystem II repair cycle. Biochem J; 406(3): 415-425
- Product Citations
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Selected references: Zienkiewicz et al. (2013).Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plan Physiol Biochem. March 16. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
AS05 084 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | cellular [compartment marker] of thylakoid membrane
Benefits of using this antibody