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Anti-SVR4-like | Supressor of variegation 4 - like
AS13 2726 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Hordeum vulgare
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SVR4-like protein sequence, UniProt: Q84RF5, TAIR: At2g31840
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 40 | 42 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Horderum vulgare
Predicted reactivity:
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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application example
SVR4-like antibody tested on intact chloroplasts isolated from ome week old barley. Intact chloroplasts were isolated from 1 week old Hordeum vulgare plants. The sample corresponding to 4ug Chl and was separated on 12% Criterion XT Bis-Tris SDS-PAGE (BioRad) gels and blotted for 25min 100V to PVDF membrane. Blot was blocked with 5% fat free skimmed milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight with agitation in 4˚C. The antibody solution was decanted and the blot was washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (swine anti-rabbit IgG horse radish peroxidase conjugated, from Dako) diluted to 1: 5000 in 1% fat free skimmed milk in PBS-T for 1h at RT with agitation. The blot was washed 5 min in PBS-T and 1 min in PBS developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 20 min.
Courtesy of Dr. Małgorzata Powikrowska, University of Copenhagen, Danmark - Additional Information
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Additional information: This protein is present in very low amounts only in early stages of plant development and this has to be taken into account when harvesting the tissue.
Western blots were done on: Arabidopsis thaliana total protein extract from cotyledons and Hordeum vulgare (2-14 days old plants). - Background
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Background: SVR4-like (Supressor of variegation 4-like) is a homolog of SVR4. It is a nuclear-encoded chloroplast-located protein required for proper function of the plastid transcriptional machinery.
Synonymes: MRL7-like, MESOPHYLL-CELL RNAI LIBRARY LINE 7-LIKE, MRL7-L. - Product Citations
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Selected references: Powikrowska et al. (2013). SVR4 of variegation 4 and SVR4-like two proteins with a role in proper organization of the chloroplast genetic machinery. Physiol Plant. Sep 23. doi: 10.1111/ppl.12108. - Protocols
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