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Anti-SPS | Sucrose phosphate synthase, global
AS03 035A | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for di and monocots | cellular [compartment marker] of cytoplasm for photosynthetic tissues.
Benefits of using this antibody
- Data sheet
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- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from conserved region within plant SPS protein sequences, including Arabidopsis thaliana isoforms 1F Q94BT0, 2F, 3F and 4F. Oryza sativa Q67WN8, Solanum tuberosum Q43845
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 25 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000-1 : 5000 (WB) Expected | apparent MW: 120 | 120-130 kDa (fragments of 30-90 kDa may be detected)
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Brassica napus, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum penelli, Miscanthus x giganteus, Pinus strobus, Solanum tuberosum, Triticum aestivum, Pinus strobus, Zea mays Predicted reactivity: Citrus sinensis, Glycine max, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Populus balsamifera, Robinia pseudoacaci, Ricinus communis, Saccharum officinarum, Solanum lycopersicum, Theobroma cacao, Vicia faba, Vitis vinifera , Species of your interest not listed? Contact us Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
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10 µg of total leaf protein from (1) A.thaliana,(3) Zea mays and (4) Hordeum vulgare extracted with PEB (AS08 300) as well as 10 µg cytosolic protein from (2) A.thaliana were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS03 035A, 1:2000, 1h) and secondary anti-rabbit (1:20 000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (90s, high sensitivity).
- Additional Information
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Additional information (application): Peptide used to elicit anti-SPS antibodies is perfectly conserved in all isoforms of SPS in plants - Background
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Background: SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.
- Product Citations
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Selected references: Bilska-Kos et al. (2020). Sucrose phosphate synthase (SPS), sucrose synthase (SUS) and their products in the leaves of Miscanthus× giganteus and Zea mays at low temperature. Planta . 2020 Jul 16;252(2):23. doi: 10.1007/s00425-020-03421-2.
Chen et al. (2018). TIC236 links the outer and inner membrane translocons of the chloroplast. Nature. 2018 Dec;564(7734):125-129. doi: 10.1038/s41586-018-0713-y.
Zhang et al. (2014). Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development. FEBS Lett. 2014 Aug 27. pii: S0014-5793(14)00621-8. doi: 10.1016/j.febslet.2014.08.019. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Katarzyna Hura | 2013-10-30Annals of Botany 101: 689-699, 2008
Accessories
AS03 035-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for di and monocots | Cellular [compartment marker] of cytoplasm for photosynthetic tissues.
Benefits of using this antibody
AS03 035-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for di and monocots | cellular [compartment marker] of cytoplasm for photosynthetic tissues.
Benefits of using this antibody
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)