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Anti-SNCA | Alpha-synuclein
AS08 358 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human
- Product Info
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Immunogen: recombinant, full length, human alpha-synuclein UniProt: P37840, epitope was mapped between amino acid 1-15 Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 100 µl Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Immunolocalization (IL), Western blot (WB) Recommended dilution: 1 : 3000 (ELISA), 1-2 µg/ml (IL), 1 : 1000 (WB) Expected | apparent MW: 14 kDa
- Reactivity
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Confirmed reactivity: Human Predicted reactivity: Primates, Chimpanzee, Gorilla - Application Examples
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Western analysis
• Membrane: Nitrocellulose
• Blocking buffer: 5% dry milk in PBS, 0.15% tween 20
• Antibody dilution: 1:1000
• Secondary antibody: anti-rabbit (HRP)
• Detection: Enhanced Chemoluminescence (pico) 10s
1. Cell lysate lacking alpha-synuclein 10 000 ng (negative control)
2. Human plasma 10 000 ng (negative control)
3. Human alpha-synuclein 100 ng
4. Human alpha-synuclien 10 ng - Additional Information
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Additional information: Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.
This antibody will recognize human SNCA monomers and multimers in Western blot and can be used to detect fibrills in a sandwich ELISA.
In ELISA this antibody can be used for detection, combined with AS13 2719 as a capture antibody. - Background
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Background: Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy.
- Product Citations
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Selected references: Bargar et al. (2021) Discrimination of MSA-P and MSA-C by RT-QuIC analysis of olfactory mucosa: the first assessment of assay reproducibility between two specialized laboratories. Mol Neurodegener. 2021 Dec 11;16(1):82. doi: 10.1186/s13024-021-00491-y. PMID: 34895275; PMCID: PMC8665327.
Brännström et al. (2014). A generic method for design of oligomer-specific antibodies. PLoS One. 2014 Mar 11;9(3):e90857. doi: 10.1371/journal.pone.0090857. eCollection 2014. - Protocols
- Antibody protocols
- Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS13 2718 | Clonality: monoclonal | Host: Mouse | Reactivity: Human
AS13 2719 | Clonality: monoclonal | Host: Mouse | Reactivity: Human