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Anti-Sec6 | Exocyst complex subunit
AS13 2686 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Sec6, UniProt F4IA34, TAIR AT1G71820
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 88.4 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Carica papaya, Medicago truncatula, Ricinus communis
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example 
50 µg of total protein from Arabidopsis thaliana, wt whole plant extract of one week old seedlings (1), whole plant extract of one week old seedlings of Arabidopsis thaliana Col-0 expressing SEC6-GFP (2), whole plant extract of one week old seedlings of Arabidopsis thaliana sec8 k.o. mutant complemented by SEC8-GFP (3), extracted with SEC6/8 buffer (20mM HEPES, pH 6.8; 150mM NaCl; 1mM EDTA; 1mM DTT; 0.5% Tween 20) were separated on 10% SDS-PAGE then using semi-dry transfer blotted 1h to the nitrocellulose membrane (1.5 mA/cm2). Blots were blocked with 5% non-fat milk in PBS overnight at 4°C with agitation. Blot was incubated in the primary antibody in 5 % milk in PBS at a dilution of 1: 7500 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed five times 5 min with PBST (PBS + 0.5% Tween 20) at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:15 000 in 5% milk in PBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to the manufacturer's instructions. Exposure time was 10 seconds.
Courtesy of Dr. Michal Hála, UEB, Czech Republic - Additional Information
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Additional information (application): Antibody is also recognizing recombinant Sec6 - Background
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Background: Sec6 is a member of the exocyst complex involved in tethering vesicles to the plasma membrane during regulated or polarized secretion.
- Product Citations
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Selected references: Kulich et al. (2018). Deubiquitinase OTU5 affects Root Responses to Phosphate Starvation. Plant Physiol. 2018 Jan 4. pii: pp.01693.2017. doi: 10.1104/pp.17.01693. - Protocols
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Accessories
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Benefits of using this antibody