Anti-RPT4a | 26S proteasome AAA-ATPase subunit RPT4a

Name: Anti-RPT4a | 26S proteasome AAA-ATPase subunit RPT4a
SKU: AS19 4263
Price: 319 EUR
Availability: InStock

Product no: AS19 4263

AS19 4263 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays

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DATA SHEET IN PDF

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Product Info

Immunogen:	Full-length recombinant RPT4a of Arabidopsis thaliana, UniProt: Q9SEI3-1, TAIR: At5g43010, overexpressed in E.coli
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
Reconstitution:	For reconstitution add 50 µl of sterile water
Storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years); once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 3000 (WB)
Expected \| apparent MW:	44.8 \| 44 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana, Zea mays
Predicted reactivity:	Actinidia chinensis, Artemisia annua, Capsicum chinense, Cicer arietinum, Helianthus annuus, Medicago truncatula, Nicotiana sylvestris, Noccaea tabacum, Solanum tuberosum, Trifolium pratense, Ricinus communis, Vigna radiata Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Additional Information
Additional information (application): This antibody is also recognizing RPT4b isofrom
Background

Background:

RPT4a (26S proteasome AAA-ATPase subunit RPT4a) is involved in positive regulation of RNA polymerase II transcriptional preinitiation complex assembly, ubiquitin-dependent ERAD pathway, ubiquitin-dependent protein catabolic process.

Alternative names: 26S proteasome subunit 10B homolog A, Regulatory particle triple-A ATPase subunit 4a, 26S proteasome regulatory subunit 10B homolog A.

Product Citations

Selected references:

Xie et al. (2024). Proteasome resides in and dismantles plant heat stress granules constitutively. Mol Cell. 2024 Sep 5;84(17):3320-3335.e7. doi: 10.1016/j.molcel.2024.07.033.
Marshall et al. (2015). Autophagic Degradation of the 26S Proteasome Is Mediated by the Dual ATG8/Ubiquitin Receptor RPN10 in Arabidopsis. Mol Cell. 2015 Jun 18;58(6):1053-66. doi: 10.1016/j.molcel.2015.04.023.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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