Anti-RPN5a | 26S proteasome regulatory subunit, putative (RPN5)

Name: Anti-RPN5a | 26S proteasome regulatory subunit, putative (RPN5)
SKU: AS19 4265
Price: 319 EUR
Availability: InStock
Rating: 5 (1 reviews)

Product no: AS19 4265

AS19 4265 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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DATA SHEET IN PDF

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Product Info

Immunogen:	Recombinant RPN5a of Arabidopsis thaliana UniProt: F4KFD7-1, TAIR: At5g09900, overexpressed in E.coli, purified from a gel piece
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
Reconstitution:	For reconstitution add 50 µl, of sterile water
Storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 5000 (WB)
Expected \| apparent MW:	52.7 \| 50 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana
Predicted reactivity:	Glycine soja, Gossypium arboreum, Juglans regia, Morus notabilis, Noccaea caerulescens, Theobroma cacao Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Additional Information

Additional information (application):

This antibody is also recognizing RPN5b.

Recommended protein load is 20 µg/well, primary antibody dilution: 1: 1000 ON/4°C and optimisation based on obtained result regarding signal/noise ratio.

Background

Background:

RPN5a | 26S proteasome regulatory subunit, putative (RPN5) is one of two isoforms for the 26S proteasome regulatory protein (RN) subunit RPN5. For many functions it acts redundantly with the paralogous gene RPN5b but also appears to exert independent effects.

Product Citations

Selected references:

Xie et al. (2024). Proteasome resides in and dismantles plant heat stress granules constitutively. Mol Cell. 2024 Sep 5;84(17):3320-3335.e7. doi: 10.1016/j.molcel.2024.07.033.
Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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Number of reviews: (1)

Jun Lee | 2023-07-28

0 | 0

-15 ug of total protein from Arabidopsis thaliana leaves
-Primary antibody at a dilution of 1:1000 o/n at 4C
-Secondary antibody (AS09 602) at 1:50000 dilution for 1hr at RT
-Detect HRP using SuperSignal West Pico PLUS