IKP

Anti-Rhamnogalacturonan-Ib (clone CCRC-M61)

Product no: AS16 3237-1ml

AS16 3237-1ml | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Arabidopsis thaliana, Sinapsus sp.

From the laboratory of Michael G. Hahn, PhD, University of Georgia

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Delivery:  3-6 business days
  • Product Info
  • Sub class: IgM
    Immunogen: MeBSA-conjugated Sinapus alba seed mucilage.
    Host: Mouse
    Clonality: Monoclonal
    Purity: Cell culture supernatant.
    Format: Liquid
    Quantity: 1 ml
    Storage: Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
    Recommended dilution: Undiluted or at 1 : 10 (ELISA), (IF), (IHC)
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Camelina sp. , Sinapus sp.
    Predicted reactivity:
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: Exact working dilution needs to be determined by end user
    Additional information (application): CCRC-M61 binds to Camelina seed mucilage and weakly to Glucuronoxylan
  • Background
  • Background: Rhamnogalacturonans (RGs) are pectic polysaccharides found in the cell wall, which contain a repeating dissacharides backbone:
    α-D-GalpA-(1,2)-α-L-Rhap-(1)
  • Product Citations
  • Selected references: Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
    Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
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