Anti-RAD23b | Rad23 UV excision repair protein family

Name: Anti-RAD23b | Rad23 UV excision repair protein family
SKU: AS19 4270
Price: 319 EUR
Availability: InStock

Product no: AS19 4270

AS19 4270 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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DATA SHEET IN PDF

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Product Info

Immunogen:	Full-length, HIS-tagged recombinant Rad23b of Arabidopsis thaliana protein sequence UniProt: F4IF85-1, TAIR: At1g79650, expressed in E.coli and purified under native conditions
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
Reconstitution:	For reconstitution add 50 µl, of sterile water
Storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 3000 (WB)
Expected \| apparent MW:	42.4 \| 55 kDa

Reactivity
Confirmed reactivity: Arabidopsis thaliana

Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information
Additional information (application): This antibody is also recognizing other RAD23 isoforms
Background

Background:

RAD23b (Rad23 UV excision repair protein family) belongs to radiation Sensitive23 (RAD23) family. Other isoforms are coded by: AT1G16190(RAD23A), AT1G79650(RAD23B), AT3G02540(RAD23C), AT5G38470(RAD23D). RAD23 proteins play an essential role in the cell cycle, morphology, and fertility of plants through their delivery of UPS (ubiquitin/26S proteasome system) substrates to the 26S proteasome.

Alternative names: Rad23 UV excision repair protein family, RAD23B.

Product Citations

Selected references:

Farmer et al. (2010). The RAD23 family provides an essential connection between the 26S proteasome and ubiquitylated proteins in Arabidopsis. Plant Cell. 2010 Jan;22(1):124-42. doi: 10.1105/tpc.109.072660.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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