Rabbit anti-Rat IgG (H&L), FITC conjugated
- Product Info
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Immunogen: Purified rat IgG (H&L) Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified rabbit IgG. Format: Liquid Quantity: 1 mg Storage: Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard. Recommended dilution: The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications - Additional Information
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Additional information: Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins
- Background
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Background: Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to FITC, which binds to anti-rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm - Product Citations
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Selected references: Kolbert et al. (2018). Nitro-oxidative stress correlates with Se tolerance of Astragalus species. Plant Cell Physiol. 2018 May 25. doi: 10.1093/pcp/pcy099.
- Protocols
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