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Rabbit anti-Mouse IgG (H&L), ALP conjugated
AS09 629 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L)
- Product Info
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Immunogen: Purified Mouse IgG, whole molecule, Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified rabbit IgG. Format: Liquid, clear, colorless. Quantity: 1 mg Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) Recommended dilution: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB) - Reactivity
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Confirmed reactivity: Mouse IgG heavy and light chains (H&L) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.
Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)
Additional information (application): Based on IEP, this antibody binds to:
heavy chains on mouse IgG
light chains on all mouse immunoglobulins
No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis. - Background
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Background: Rabbit anti-mouse IgG (H&L) is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all mouse immunoglobulins in immunological assays.
- Protocols
- Antibody protocols
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