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Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, HRP conjugated
AS10 1045 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Goat IgG (H&L)
- Product Info
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Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified IgG, F(ab)'2 fragment. Format: Lyophilized Quantity: 0.5 mg Reconstitution: For reconstitution add 0.55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1 : 500-1 : 5000 (IHC),1 : 200 -1 : 5 000 (WB) - Additional Information
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Additional information: This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, Protease/IgG free and 0.1% (v/v) Kathon CG is used as preservative.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. - Background
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Background: Rabbit anti-goat IgG (H&L),HRP conjugated is a F(ab)'2 fragment of a secondary antibody which binds to all goat IgGs in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG. - Protocols
- Antibody protocols
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