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Anti-PRP40B | pre-mRNA-processing protein 40B
AS14 2785 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: Recombinant Arabidopsis thaliana PRP40b, UniProt:F4JCC1, TAIR: At3g19670
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 2000 (WB) Expected | apparent MW: 113 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana
Predicted reactivity: Brassica rapa
Species of your interest not listed? Contact usNot reactive in: - Application Examples
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Application example 
Total protein from rosette leaves, 30 ug of Arabidopsis thaliana whole leaf extract (prp40ab) (1), 30 ug of Arabidopsis thaliana whole leaf extract (wild-type) (2), extracted with buffer (100mM Tris, 10% glicerol, 5mM EDTA, 5mM EGTA, 0.15M NaCl, 0.75% Triton X100, 0.05% SDS, 1mM DTT) and denatured with 0.06M Tris-HCl, 5% glycerol, 2% SDS, 4% β-mercaptoethanol, 0.0025% bromophenol blue at 95 C for 5 min were separated on 8 % SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 5% low-fat dried milk in TBS + 0,1%Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation in 2% low-fat dried milk in TBS + 0,1%Tween. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in 2% low-fat dried milk in TBS + 0,1%Tween for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL of high sensitivity. Exposure time was 60 seconds.
MW markers: PageRuler Plus Prestained (ThermoFisher)
Courtesy of Agata Stępień, Adam Mickiewicz University, PolandApplication examples: 
Reactant: Arabidopsis thaliana (Thale cress)
Application: Western Blotting
Pudmed ID: 36087009
Journal: Plant Cell
Figure Number: 1D
Published Date: 2022-11-29
First Author: Stepien, A.
Impact Factor: 7.018
Open PublicationAtPRP40 regulates the association of SE with RNAPII. A, Close proximity of AtPRP40b and SE in the cell nucleus (first image, magenta signals) analyzed by PLA in view of SE nuclear localization (second image, green signals). DNA was stained with Hoechst (blue). Scale bar = 2.5 µm. B, Close proximity of SE and RNAPII phosphorylated at CTD Ser5 or Ser2 in WT, prp40ab, and se-2 plants (first columns, magenta signals) in view of P-Ser5-RNAPII or P-Ser2-RNAPII nuclear localization (second columns, green signals) detected by PLA. DNA was stained with Hoechst (blue). Scale bar = 2.5 µm. C, Close proximity of AtPRP40b and RNAPII phosphorylated at CTD Ser5 or Ser2 in WT, se-2, and prp40ab plants (first columns, magenta signals) in view of P-Ser5-RNAPII or P-Ser2-RNAPII nuclear localization (second columns, green signals) detected by PLA. DNA was stained with Hoechst (blue). Scale bar = 2.5 µm. D, In vitro pull-down assays using recombinant AtPRP40b and SE proteins (both full length and the shortened C-terminus variant Δ681–720 were used) and biotinylated CTD peptides in unphosphorylated (UnP) or phosphorylated Ser5 (P-Ser5-CTD) or Ser2 (P-Ser2-CTD) forms. The recombinant proteins were incubated with CTD peptides immobilized on beads. The obtained complexes were washed, eluted, and analyzed by immunoblot. Input represents 1/10 of the protein sample.
- Background
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Background: PRP40B (pre-mRNA-processing protein 40B) protein that binds the carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II and functions as a scaffold for RNA processing machineries. Ubiquitously expressed and localized to the nucleus.
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS14 2784 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana