Anti-PRO4,5 | Profilin-4,5 (clone mAbPRF45a (2-B8))
AS16 3143 | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: Profilin 4 (PRF4) from Arabidopsis thaliana, UniProt: Q38905, TAIR: AT4G29340
Sub class: IgG1 Host: Mouse Clonality: Monoclonal Purity: IgG1 immunoglobulin purified on Protein G in 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) sodium azide. Format: Liquid Quantity: 100 µg Storage: Store at -20°C,Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunohistochemistry (IHC), Western blot (WB) Expected | apparent MW: 14 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
5 µg of recombinant profilins from Arabidopsis thaliana and 20 µg of total protein from whole plant extract. Coomassie blue stained gel (bottom) shows equal loading of recombinant profilins. - Additional Information
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Additional information: Exact working dilution needs to be determined by end user Additional information (application): This antibody reacts with both, PRF4 (profilin 4) and PRF5 (profilin 5) - Background
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Background: Profilin is an actin monomer-binding protein, involved in the organization and reconstruction of the actin cytoskeleton in eukaryotes. Profilin also interacts with membrane polyphospho-inositides, the Arp 2/3 complex, short proline rich stretches, and other ligands. - Product Citations
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Selected references: Kandasamy, M.K., et al. (2002). Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues. Cell Motil Cytoskeleton 52(1):22-32.
McKinney, E.C., et al. (2001) Small changes in the regulation of one Arabidopsis profilin isovariant, PRF1, alter seedling development. Plant Cell 13(5): 1179-91. - Reviews:
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Accessories
AS16 3142 | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana
AS13 2640 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stoloniferacv. ‘Penncross’,Arabidopsis thaliana, Brassica sp., Cannabis sativa L., Cucumis sativus, Cynara cardunculus, Glycine max, Hordeum vulgare, Nicotiana tabacum, Phaseolus vulgaris, Phoenix dactylifera, Picrorhiza kurroa, Setaria italica, Solanum tuberosum, Triticum aestivum, Zea mays