IKP

Anti-PRO4,5 | Profilin-4,5 (clone mAbPRF45a (2-B8))

Product no: AS16 3143

AS16 3143  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Arabidopsis thaliana

421 €
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DATA SHEET IN PDF
Delivery:  7-10 business days
  • Product Info
  • Immunogen: Profilin 4 (PRF4) from Arabidopsis thaliana, UniProt: Q38905, TAIR: AT4G29340
    Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: IgG1 immunoglobulin purified on Protein G in 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) sodium azide.
    Format: Liquid
    Quantity: 100 µg
    Storage: Store at -20°C,Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunohistochemistry (IHC), Western blot (WB)
    Expected | apparent MW:

    14 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    western blot using anti-profilin 4 antibodies

    5 µg of recombinant profilins from Arabidopsis thaliana and 20 µg of total protein from whole plant extract. Coomassie blue stained gel (bottom) shows equal loading of recombinant profilins.
  • Additional Information
  • Additional information: Exact working dilution needs to be determined by end user
    Additional information (application): This antibody reacts with both, PRF4 (profilin 4) and PRF5 (profilin 5)
  • Background
  • Background: Profilin is an actin monomer-binding protein, involved in the organization and reconstruction of the actin cytoskeleton in eukaryotes. Profilin also interacts with membrane polyphospho-inositides, the Arp 2/3 complex, short proline rich stretches, and other ligands.
  • Product Citations
  • Selected references: Kandasamy, M.K., et al. (2002). Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues. Cell Motil Cytoskeleton 52(1):22-32.
    McKinney, E.C., et al. (2001) Small changes in the regulation of one Arabidopsis profilin isovariant, PRF1, alter seedling development. Plant Cell 13(5): 1179-91.
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