Diatoms


Below you will find antibodies with confirmed or predicted reactivity to Diatoms.


Suggested Control Antibodies for Diatoms

AtpB | beta subunit of ATP synthase, AS05 085 | Clonality: polyclonal | Host: rabbit 
This antibody works well and detects both the chloroplastic and the mitochondrial forms of the enzyme. It usually gives a tight band and shows good reactivity. With ECL Advance detection we usually load 1 ug of total protein from diatom extracts, using this antibody.

RbcL | Rubisco large subunit, form I, AS03 037 | Clonality: polyclonal | Host: rabbit 
This antibody is very 'hot' and the RUBISCO target is abundant, so only a small load of total protein is required to get a total band. With ECL Advance we usually load 0.5 or even 0.25 ug of total protein from diatom extracts, using this antibody. So depending upon the strength of the antibody to which you are comparing, this anti-RUBISCO may be too 'hot' to be a good comparison.

PsbA | D1 protein of PSII, C-terminal, AS05 084 | Clonality: polyclonal | Host: rabbit
This antibody is used if the comparison protein is a hydrophobic membrane protein. This antibody works well but the PsbA protein is subject to fragmentation upon storage and freeze-thaw cycles. This might be a good index of the 'quality' of the total protein extracts. For this antibody we normally load 1 ug total protein, when detecting using a sensitive chemiluminescent detection system like Agrisera ECLBright (extreme low femtogram range).

Protocol for protein extraction from diatoms 



Protocols and Technical Information  •  Support  •  Western Blot Video Tutorials
 Agrisera antibodies Diatoms

 

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AS12 2111 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Z. mays, C. reinhardtii, S. lycopersicum

329 €
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AS13 2640 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stoloniferacv. ‘Penncross’,Arabidopsis thaliana, Brassica sp., Cannabis sativa L., Cucumis sativus, Cynara cardunculus, Glycine max, Hordeum vulgare, Nicotiana tabacum, Phaseolus vulgaris, Phoenix dactylifera, Picrorhiza kurroa, Setaria italica, Solanum tuberosum, Triticum aestivum, Zea mays

329 €
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AS13 2669 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants

Benefits of using this antibody

329 €
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AS13 2671 | Clonality: Polyclonal | Host: Chicken | Reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae cellular [compartment marker] for plasma membrane

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329 €
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AS14 2775 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Vigna radiata

329 €
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AS15 2873S |  protein standard for quantitation and positive control

185 €
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AS15 2955 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:  photosynthetic bacteria, archea, dinoflagellates

Benefits of using this antibody

329 €
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AS15 2991 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, Scenedesmus sp.

329 €
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AS15 2992 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

329 €
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AS15 2994 | Reactivity: Rubisco protein

This set enables quantification of Rubisco using ELISA in samples from a range of various species.

1050 €
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AS16 3976  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Brassica oleracea var. botrytis cv. ’Diadom, [compartment marker] of mitochondrial inner membrane

Benefits of using this antibody

329 €
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AS17 4115 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Cyclotella sp.Thalassiosira pseudonana

329 €
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AS17 4116 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Heterosigma akashiwo

329 €
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AS18 4202-1ml | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
268 €
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By Dr. Dmitry Shevela, Prof. Lars Olof Björn, Prof. Govindjee
Hardcover: 204 pages Publisher: World Scientific Publishing Company (7 Jan. 2019)
Language: English
ISBN-10: 9813223103 ISBN-13: 978-9813223103

Product Number: AS19 4290Book ; Product Dimensions: 15.2 x 1.9 x 22.9 cm

AS19 4303  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Phaeodactylum tricornutum

164.50 € (329 €)
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AS19 4367 | Clonality:Polyclonal | Host: Rabbit | Reactivity: Pheodactyllum tricornutum

329 €
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SA000001  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants and algae

This set includes three discounted, well published, compartment marker antibodies of your choice. Benefits of using this antibody set

Related products:
SA000002 | Plant Cell Compartment Antibody Marker Set | 5 antibodies
SA000003 | Plant Cell Compartment Antibody Marker Set | 10 antibodies
786 €

SA000002  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants and algae

This set includes five discounted, compartment marker antibodies of your choice. Benefits of using this antibody set

Related products:
SA000001 | Plant Cell Compartment Antibody Marker Set | 3 antibodies
SA000003 | Plant Cell Compartment Antibody Marker Set | 10 antibodies

1319 €
SA000003  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants and algae

This set includes ten discounted, compartment marker antibodies of your choice. Benefits of using this antibody set

Related products:
SA000001 | Plant Cell Compartment Antibody Marker Set | 3 antibodies
SA000002 | Plant Cell Compartment Antibody Marker Set | 5 antibodies

2467 €
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