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Anti-PIP (PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5) aquaporins, Biotin-conjugated (40 ”g)
AS09 487B | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, J. curcas, P. nigra, P. trichocarpa, R. sativus
- Product Info
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Immunogen: KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana: PIP1;1 P61837,At3g61430 PIP1;2 Q06611,At2g45960 PIP1;3 Q08733,At1g01620 , PIP1;4 Q39196, At4g00430, PIP1;5 Q8LAA6 At4g23400
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin. Format: Liquid Quantity: 40 µg Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 30.68 | 28 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Brassica sp., Jatropha curcas L. cv. Biji Jarak , Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Thellungiella salsuginea
Predicted reactivity: Brassica sp., Hordeum vulgare, Juglans regia, Oryza sativa, Populus tremula, Triticum aestivum, Vicia faba
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: Antibodies will detect target protein in a few µg of a crude preparation loaded per well, If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient Additional information (application): Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
- Background
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Background: PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinistic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. ShevelaMethod for isolation of plant plasma membranes
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Courtesy of Dr. Masayoshi Maeshima, Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences Nagoya University Nagoya, Japan
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Accessories
AS09 487-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, J. curcas, P. nigra, P. trichocarpa, R. sativus
AS09 489 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana tabacum, Phoenix dactilifera, Raphanus sativus, Zea mays
Replaced by AS22 4816
AS09 490 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Raphanus sativus
Replaced by AS22 4813