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Anti-Pectic polysaccharide, beta-1,4-galactan (monoclonal, clone LM5)
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
- Product Info
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Immunogen: Pectic polysaccharide (beta 1,4-galactan) Sub class: IgG Host: Rat Clonality: Monoclonal Purity: Cell culture supernatant. Format: Liquid Quantity: 1 ml Storage: Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Immunoflourescence (IF) Recommended dilution: 1:10 (ELISA, IF) - Reactivity
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Confirmed reactivity: Higher plants, ferns and mosses Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Samples:
A - Lignin autofluorescence
B - LM5 staining of wood
C - Composite
D - LM5 antibody staning + brightfield
Objective: 20x,100 µm scalebar
Type of material: Pine (Pinus sylvestris) reaction wood section in paraffin cut with microtome
Fixation: none
Hydrophilization: no
Cell wall digestion: no
Membrane permeabilization: none Antigen retrieval: no
Blocking buffer: 5% milk in PBS
Washing buffer: 5% milk in PBS-T
Primary antibody dilution and incubation time: 1:20 incubation 90 minutes, room temperature
Secondary antibody dilution and incubation time and supplier: 1:100 Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 (invitrogen: A-11007), incubation for 60 minutes in room temperature.
Co-staining of the nucleus (DAPI): no
Cell wall and nucleus staining: none
Courtesy of Dr. Jan Łyczakowski, Jagiellonian University, Kraków, Poland - Additional Information
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Additional information: Contains 0.05% Sodium Azide.
No cross-reactivity with (1-3)-beta-D-galactans or (1-6)-beta-D-galactans.
It recognizes a linear tetrasaccharide in (1-4)-beta-D-galactans.
In ELISA (competitive inhibition), antibody is binding to: (1-4)-beta-D-galactan was inhibited (50%) by 58 µg/ml (1-4)-beta-D-galactotetraose and by 0.7 µg/ml lupin (1-4)-beta-D-galactan. - Background
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Background: The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.
Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.
- Product Citations
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Selected references: Andersen et al. (2016). Characterization of the LM5 pectic galactan epitope with synthetic analogues of ß-1,4-d-galactotetraose. Carbohydr Res. 2016 Dec ;436:36-40.doi: 10.1016/j.carres.2016.10.012.
Jones et al. (1997). Development and validation of an in vitro model system to study peripheral sensory neuron development and injury. Sci Rep. 2018 Oct 29;8(1):15961. doi: 10.1038/s41598-018-34280-3. - Reviews:
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From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
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