1
Anti-NtrC | Thioredoxin reductase (TR/TRxR) (algal)
AS06 159 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
- Product Info
-
Immunogen: KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 200 µg Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 500 (WB) Expected | apparent MW: 55.3 | 49 kDa (following processing) - Reactivity
-
Confirmed reactivity: Chlamydomonas reinhardtii Predicted reactivity: Chlamydia sp., Marchantia polymorpha, Nannochloropsis gaditana, Ostreococcus luminarius, Physcomitrium patens, Synechococcus sp.
Species of your interest not listed? Contact usNot reactive in: Arabidopsis thaliana
- Additional Information
-
Additional information: Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria
Additional information (application): Antibody will react with long NtrC of Chlamydomonas reinhardtii proteins and shorter NtrA-type rpoteins, which would be distinguished by migration size. - Background
-
Background: Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC 1.8.1.9) which is reducing thoredoxin (Trx). Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis thaliana and Chlmydomonas reinhardtii NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein size of ca. 488 amino acids.
- Protocols
-
Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
-
Ayten Ulusan | 2017-05-08The item is very good.
Accessories
AS07 243 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)