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Anti-NtcA | Global nitrogen regulator
AS12 1873 | Clonality: Polyclonal | Host: Rabbit | Reactivity: cyanobacteria
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from known cyanobacterial NtcA protein sequences including UniProt: P33779
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 200 µg Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 25 kDa
- Reactivity
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Confirmed reactivity: Synechocystis sp. PCC6803 Predicted reactivity: Anabaena sp., Gleobacter sp., marine Synechococcus strains, Trichodesmium sp.
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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application example
5 µg of total protein from Synechocystis sp. PCC 6803 extracted with PEB were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 32 seconds.
- Background
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Background: NtcA acts as a transcriptional activator of genes subjected to nitrogen control.
- Product Citations
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Selected references: Ge at al. (2017). Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017 Jul;16(7):1258-1274. doi: 10.1074/mcp.M116.068080. - Protocols
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- Reviews:
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Ajlani | 2015-07-08Works well, no background or unspecific bands in our hands (Synechocystis)
Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS01 021A | Clonality: Polyclonal | Host: Hen | Reactivity: [global antibody] for bacteria
Benefits of using this antibody
AS01 021S | Recombinant protein standard for quantitation of Nifh protein