Anti-Na+/H+ antiporter, sodium/hydrogen exchanger

Western blot analysis of protein expression of plasma membrane (PM) H+-ATPase and Na+/H+ antiporter (NHE-1).Shown in (A) and (C) are the relative protein expression level of K. obovata seedlings grown under 400 mM NaCl and various concentrations of SNP for 15 days. Shown in (B) and (D) represent the relative protein expression level of K. obovata seedlings treated with the following Hoagland solution-based solutions for 15 days: Control (only Hoagland solution), NaCl (400 mM), SNP (100 µM), NaCl+SNP (400 mM NaCl+100 µM SNP), cPTIO+L-NNA (200 µM cPTIO+100 µM L-NNA), NaCl+cPTIO+L-NNA (400 mM NaCl+200 µM cPTIO+100 µM L-NNA). Three independent experiments were performed. The relative intensity of bands (column charts) was quantified by densitometric analysis with Quantity One software and expressed relative to the intensity of the control band defined as 1. For each parameter, bars with different letters indicate significant difference at P<0.05.

Western-blot analysis of root PM H+-ATPase(a) and vacuolar Na+/H+ antiporter (NHE1) (c) of barley seedlings treated with 150?mM NaCl and various NaHS concentrations (0, 50, 100, 200 and 500??M) for 48?h. CK: Hoagland solution only without adding NaHS and NaCl. Three independent experiments were conducted, and the immunoblotting results show similar trends of protein expression. The relative protein expression level is shown as the ratio of PM H+-ATPase to ?-actin (b) and NHE1 to ?-actin (d) analyzed with Quantity One software. Mean values?±?SE were calculated from independent experiments and columns labeled with different letters are significantly different at P?

Reactant: Hordeum vulgare (Barley)

Application: Western Blotting

Pudmed ID: 26213372

Journal: Sci Rep

Figure Number: 7C

Published Date: 2015-07-27

First Author: Chen, J., Wang, W. H., et al.

Impact Factor: 4.13

Open Publication

Western-blot analysis of root PM H+-ATPase(a) and vacuolar Na+/H+ antiporter (NHE1) (c) of barley seedlings treated with 150?mM NaCl (Na), 150?mM NaCl+100??M NaHS (Na+S), 150?mM NaCl+100??M NaHS+200??M cPTIO (Na+S+c), 150?mM NaCl+100??M SNP (Na+N), 150?mM NaCl+100??M SNP+200??M cPTIO (Na+N+c) for 48?h, respectively. CK: Hoagland solution only without adding NaHS and NaCl. Three independent experiments were conducted, and the immunoblotting results show similar trends of protein expression. The relative protein expression level is shown as the ratio of PM H+-ATPase to ?-actin (b) and NHE1 to ?-actin (d) analyzed with Quantity One software. Mean values?±?SE were calculated from independent experiments and columns labeled with different letters are significantly different at P?<?0.05.