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Anti-NDPK | Nucleaoside diphosphate kinase
AS08 302 | Clonality: Polyclonal | Host: Rabbit | Reactivity:A.thalina,P.sativum
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Pisum sativum NDPK, UniProt: Q9SP13. Peptide is conserved in Arabidopsis thaliana NDPKIII, UniProt:O49203 and NDPK IV, UniProt: Q8LAH8, but is not conserved in NDPK1.
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 5000 (WB) Expected | apparent MW: 25 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Pisum sativum Predicted reactivity: Brassica campestris, Oryza sativa, Spinacia oleracea, Vitis vinifera
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information (application): 1: 5000 dilution in a western blot was used for 15 µg of protein per lane
- Background
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Background: Nucleoside diphosphate kinase protein (EC=2.7.4.6) is catalysing the transfer of a g-phosphate group from adenosine triphosphate (ATP) to a cognate nucleoside diphosphate. This contributes to balancing of the nucleoside pool. NDPK enzymes are present in most subcellular compartments of the eukaryotic cell.
- Product Citations
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Selected references: Hammargren et al. (2007). On the phylogeny, expression and targeting of plant nucleoside diphosphate kinases. Physiol Plant. 129: 79–89.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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Veronika Z | 2014-09-29WB was performed in 10% SDS gel. Samples of Arabidopsis thaliana extract. AB dilution 1:3000 on 10 µg of protein. Protein MW is about 20-25 kDa. Good!
Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)