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Anti-NAD-ME | Mitochondrial NAD-dependent malic enzyme
Product no: AS16 3932
AS16 3932 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Amaranthus hypochondriacus, Arabidopsis thaliana, Glycine max, Zea mays
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- Product Info
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Immunogen: Native, NAD-ME purified from mitochondria isolated from mature amaranth leaves, UniProt: P37224 Host: Rabbit Clonality: Polyclonal Purity: Serum + 0.01% sodium azide. Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunoprecipiatation (IP), Immunolocalization (IL), Western blot (WB) Recommended dilution: 1 : 25 to 1 : 100 (IL), 1 : 1000 (WB) Expected | apparent MW: 65 kDa
- Reactivity
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Confirmed reactivity: Amaranthus hypochondriacus, Arabidopsis thaliana, Flaveria sp., Glycine max, Nicotiana tabacum, Zea mays
Predicted reactivity: Amborella trichopoda, Arabis alpina, Beta vulgaris, Brachypodium distachyon, Brassica oleracea, Citrus sinensis, Coffea canephora, Cucumis sativus , Cynara cardunculus, Erythranthe guttata, Eucalyptus grandis, Eutrema salsugineum, Gossypium raimondii, Hordeum vulgare, Jatropha curcas, Kalanchoe fedtschenkoi, Malus domestica, Medicago truncatula, Morus notabilis, Oryza sativa, Phaseolus vulgaris, Physcomitrium patens, Picea sitcHensis, Populus trichocarpa, Prunus persica, Ricinus communis, Selaginella moellendorffii, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum aestivum, Zostera marina, Wollemia nobilis, Vitis vinifera
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: Antibody can be used in immunolocalization using immunogold TEM.
This antibody recognizes the large subunit (MEL) of the NAD-ME.
Immunolocalization method is described in Long et al. (1994). - Background
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Background: NAD-ME (Mitochondrial NAD-dependent malic enzyme) is an enzyme which in some C4 plants catalyzes the decarboxylation of 4 carbon malate in the bundle sheath cells, releasing carbon dioxide. This enzyme plays a key role in photosynthetic carbon fixation. - Product Citations
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Selected references: Hoa et al. (2024). Proteomic analysis on symbiotic differentiation of mitochondria in soybean nodules. Comparative Study Plant Cell Physiol. 2004 Mar;45(3):300-8. doi: 10.1093/pcp/pch035.
Long et al. (1994). Cloning and analysis of the C4 photosynthetic NAD-dependent malic enzyme of amaranth mitochondria. J Biol Chem. 1994 Jan 28;269(4):2827-33. - Protocols
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- Reviews:
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Ayten Ulusan | 2017-05-08The item is very good
Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
213 €