Anti-Lysine-tRNA ligase
AS15 2899 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Lysine-tRNA ligase protein sequence of Arabidopsis thaliana UniProt: Q39101, TAIR: AT3G01060
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 100 µl Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 500 (WB) Expected | apparent MW: 41 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana
- Application Examples
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Application example
Total soluble proteins (stroma) extracted from 0.2 g leaf by homogenization in a buffer containing 20 mMTris-HCl, pH 9.0, 250m MNaCl, 50m MNaHCO3, 4mMMgCl2, and an EDTA-free protease inhibitor cocktail (Roche). After removal of cell debris by centrifugation for 5min at 13,000g and 4°C, a volume corresponding to 10 μg of protein was loaded on12% SDS-PAGE and blotted as descried above.Courtesy of Dr. Rikard Fristedt, VU University Amsterdam, The Netherlands
- Additional Information
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Additional information (application): This protein is present in very low levels, therefore western blot conditions need to be adjusted by higher protein load/well and usage of a very sensitive detection system - Background
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Background: Lysine-tRNA ligase is located in chloroplast and involved in circadian rhytm. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
This product can be purchased in 3 different volumes:
AS16 ECL-S-10, 10 ml. Trial size limited to one per customer
AS16 ECL-S-100, 100 ml
Choose the appropriate volume in the drop down menu to the right