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Anti-Lhcb4 | CP29 (Lhcb4) homolog (Ostreococcus tauri)
AS09 512 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Coccomyxa subellipsoidea, Ostreococcus tauri
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Lhcb4 (CP29) protein sequence from Ostreococcus tauri Q3B9U8
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 200 µl Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 2000 (WB) Expected | apparent MW: 27 | 27 kDa
- Reactivity
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Confirmed reactivity: Coccomyxa subellipsoidea, Ostreococcus tauri Predicted reactivity: Bathycoccus prasinos, Micromonas sp.,
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
1.0-4.5 µg of chlorophyll from Coccomyxa subellipsoidea cells were loaded to lanes. Samples were denatured with Laemmli buffer at 75°C for 5 min and were separated on 12% SDS-PAGE and blotted 30 min to PVDF using wet transfer. Blot was blocked with 5% milk in TBS for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 overnight at 4°C with agitation in 1% milk in TBS-T. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 1% milk in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min in TBS-T and 2 times for 5 min in TBS, and developed for 1 min with 1.25 mM luminol, 0.198 mM coumaric acid and 0.009% H2O2 in 0.1 M Tris- HCl, pH 8.5. Exposure time in ChemiDoc System was 115 seconds.
Courtesy MSc Paweł Rogowski, Warsaw University, Poland - Background
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Background: Lhcb4 (CP29) is a member of the family of chlorophyll a/b-binding proteins, which is conserved in higher plants and green algae. Lhcb4 is associated with Photosystem II serving both as a light-harvesting antenna protein as well as playing a role in photoprotective dissipation of excitation energy. Alternative name: CP29-like light-harvesting chlorophyll a/b binding protein chlor (IC)
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Accessories
AS09 512 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Coccomyxa subellipsoidea, Ostreococcus tauri
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)