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Anti-Lhca5 | PSI type V chlorophyll a/b-binding protein
AS05 082 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants including A.thaliana, H.vulgare, S.oleracea
- Data sheet
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- Product Info
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Immunogen: BSA-conjugated synthetic peptide derived from the Lhca5 protein of Arabidopsis thaliana (At1g45474). This sequence is highly conserved in Lhca5 proteins of angiosperms (monocots and dicots) and gymnosperms. UniProt: Q9C639, TAIR: At1g45474
Host: Rabbit Clonality: Polyclonal Purity: Total IgG. Protein G purified in PBS pH 7.4. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000-1 : 3000 (WB) Expected | apparent MW: 27.8 | 23 for Arabidopsis thaliana
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Bryopsis corticulans, Hordeum vulgare, Spinacia oleracea Not reactive in: Chlamydomonas reinhardtii, Physcomitrella patens
- Additional Information
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Additional information: More information on the Lhca5 localization within the LHCI antenna can be found in Lucinski et al. (2006) Lhca5 interaction with plant photosystem I. FEBS Letters 580:6485-6488
Additional information (application): Protein is processed into mature form (Jansson 1999).
Urea is not recommended to use in extraction buffer and in a gel, when working with this antibody. - Background
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Background: Lhca5 is a low abundant chlorophyll a/b-binding protein that has been found to associate with Photoystem I in sub-stoechiometric amounts. Unlike the four main Lhca-types (Lhca1-4) Lhca5 seems to be able to associate with PSI or the LHCI at varying positions. The regulation pattern for the expressino of the Lhca5 gene differs from the standard Lhc pattern as expression seems to be positively regulated under light and cold stress conditions.
- Product Citations
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Selected references: Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.
Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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