Anti-KLH | Keyhole Limpet Hemocyanin (monoclonal, clone 6D12)

Name: Anti-KLH | Keyhole Limpet Hemocyanin (monoclonal, clone 6D12)
SKU: AS22 4757
Price: 399 EUR
Availability: InStock

Product no: AS22 4757

AS22 4757 | Clonality: Monoclonal | Host: Mouse | Reactivity: KLH | Keyhole Limpet Hemocyanin

399 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Sub class:	IgG1 / Kappa
Immunogen:	Hemocyanin from Megathura crenulata.
Host:	Mouse
Clonality:	Monoclonal
Purity:	Purified on Protein A to a total immunoglobulin fraction, in Tris 0.1 M, glycine 0.1 M and 2% sucrose.
Format:	Lyophilized
Quantity:	100 µg
Reconstitution:	Reconstitute in distilled water.
Storage:	Lyophilized powder is stable for a minimum of 2 years at -20°C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°C for up to a month and make aliquots for extended storage.
Tested applications:	ELISA (ELISA), Western blot (WB)
Recommended dilution:	1:10 000 - 1:100 000 (ELISA), 1: 500 - 1: 5000 (WB)
Expected \| apparent MW:	ca. 400 kDa/subunit

Reactivity
Confirmed reactivity: Reacts with Keyhole Limpet 8-9 kDa Hemocyanin protein.
Additional Information

Additional information (application):

Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide.

Background

Background:

Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

Protocols
Antibody protocols
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