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Anti-HCP | Hyper conserved protein
AS10 688 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Prochlorococcus MIT 9313
- Product Info
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Immunogen: Synechococcus WH8102 recombinant HCP
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 200 µl Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 7 kDa
- Reactivity
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Confirmed reactivity: Prochlorococcus MIT 9313 Predicted reactivity: Cyanobacteria
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example 
7ug of of total protein extract from Prochlorococcus MIT 9313 collected at various days of culture were loaded in each of the samples. The loading scheme is: Lane 1: day 9 sample, Lane 2: day 7 sample, Lane 3: day 9 sample, Lane 4: day 7 sample, Lane 5: day 5 sample, Lane 6: Ladder, Lane 7:240 fmol HCP recombinant standard, Lane 8: 120 fmol recombinant standard, Lane 9: 60 fmol recombinant standard, Lane 10: 30 fmol recombinant standard. The gel was run at 200V for 35 minutes, transferred for 45 minutes at 30V (it was the only blot in the transfer rig). And a nitrocellulose membrane was used for blotting. It was blocked overnight in Blocking Solution. A 1: 4000 dilution of primary anti-HCP antibody was used and the membrane was blocked for an hour. Then the washes in TBS-T were done - 2x briefly, 1x for 15min, and 3x for 5min. A 1:25 000 dilution of secondary antibody was used, and the membrane was blocked for another hour followed by the washes in TBS-T. Reaction was developed using extreme low femtogram chemiluminescent detection reagent. - Background
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Background: HCP (hyper conserved protein) is perfectly conserved between Prochlorococcus and Synechococcus group. So far its exact function is not known.
- Product Citations
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Selected references: Whidden et al. (2014). Quantitative and functional characterization of the hyper-conserved protein of prochlorococcus and marine synechococcus. PLoS One. 2014 Oct 31;9(10):e109327. doi: 10.1371/journal.pone.0109327. eCollection 2014.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection
- Reviews:
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Accessories
AS03 037 | Clonality: Polyclonal | Host: Rabbit | Reactivity: global antibody and compartment marker for higher plants, lichens, algae, cyanobacteria, dinoflagellates, diatoms
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AS05 084 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | cellular [compartment marker] of thylakoid membrane
Benefits of using this antibody
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
This product can be purchased in 3 different volumes:
AS16 ECL-S-10, 10 ml. Trial size limited to one per customer
AS16 ECL-S-100, 100 ml
Choose the appropriate volume in the drop down menu to the right