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Goat anti-Rat IgG (H&L), HRP conjugated
AS10 1187 | Clonality: Polyclonal | Host: Goat | Reactivity: Rat IgG (H&L)
- Product Info
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Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified rat IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily. Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Store liquid material at 2-8°C up to 6 months. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: The optimal working dilution should be determined by the investigator - Reactivity
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Confirmed reactivity: Heavy chains on rat IgG and with the light chains on all rat immunoglobulins Not reactive in: Non-immunoglobulin rat serum proteins - Application Examples
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Application examples: 
Reactant: Populus tremula (Aspen)
Application: Western Blotting
Pudmed ID: 33381142
Journal: Front Plant Sci
Figure Number: 15A
Published Date: 2021-01-01
First Author: Niittylä, T.
Impact Factor: 6.308
Open PublicationWestern blot analysis of AGPs in stem tissues of aspen. Water-extracted AGPs from 2 mg dried plant material were loaded per lane, separated in SDS-PAGE according to size and transfered onto a PVDF membrane. Epitopes were detected by different anti-AGPs mAbs (JIM8, JIM13, JIM14, JIM16, LM2, LM14, and MAC207) and anti-extensins mAbs (JIM12, JIM19, JIM20, and LM1). Molecular mass (kDa) is indicated on the left. M, marker; Phl/Cam, phloem/cambium; Dev xylem, developing xylem; Mat xylem: mature xylem.
- Additional Information
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Additional information: Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG as preservative.
- Background
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Background: Goat anti-rat IgG (H&L), HRP conjugated is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
- Product Citations
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Selected references: Saintenac et al. (2021) A wheat cysteine-rich receptor-like kinase confers broad-spectrum resistance against Septoria tritici blotch. Nat Commun. 2021 Jan 19;12(1):433. doi: 10.1038/s41467-020-20685-0. PMID: 33469010; PMCID: PMC7815785. - Protocols
- Antibody protocols
- Reviews:
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Szymon Kubala | 2017-04-25this antibody was used against primary RAT anti HA antibody. The 1:5000 dilution was performed. WB analysis was performed on the plant extract carrying the HA tagged protein
Accessories
AS10 1210 | Clonality: Polyclonal | Host: Goat | Reactivity: Rat IgG (H&L)
AS10 663 | Clonality: Polyclonal | Host: Goat | Reactivity: Rat IgG (H&L)