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Goat anti-Rabbit IgG (H&L), TRITC conjugated, min, cross-reactivity to bovine,goat,human, mouse,rat IgG
AS10 966 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
- Product Info
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Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Recommended dilution: The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt) - Additional Information
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Additional information: This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
- Background
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Background: Goat anti-rabbit IgG (H&L) is a Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated secondary antibody, which binds to all rabbit IgGs in immunological assays. It has been specially adsorbed against bovine,goat,human,mouse and rat IgG. (TRITC) has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase rabbit IgG. - Protocols
- Antibody protocols
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