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Goat anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to human serum
- Product Info
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Immunogen: Purified rabbit IgG (H&L) Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Recommended dilution: This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, - Additional Information
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Additional information: Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.
Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, human serum proteins.
- Background
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Background: Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). - Product Citations
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Selected references: Sun et al. (2019). Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment. Plant Cell. 2019 Jul;31(7):1648-1664. doi: 10.1105/tpc.18.00787.
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Accessories
AS10 1230 | Clonality: Polyclonal | Host:Goat | Reactivity: Rabbit IgG
AS10 1018 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS10 1204 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
