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Goat anti-Rabbit IgG (H&L) HRP conjugated, min,cross-reactivity to bovine/Human/mouse IgG/serum
AS10 668 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
- Product Info
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Immunogen: purified Rabbit IgG, whole molecule
Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Tested applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1 : 500 -1 : 5000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 500 -1 : 5000 (WB) - Reactivity
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Confirmed reactivity: heavy chains of Rabbit IgG, Light chains on all Rabbit immunoglobulins Predicted reactivity: Rabbit IgG Heavy and Light chains (H&L) of all Rabbit immunoglobulins Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
Additional information (application): Based on immunoelectrophoresis no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
serum from bovine, human or mouse
IgG from human or mouse
- Background
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Background: Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to rabbit IgGs in immunological assays.
- Product Citations
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Selected references: Ghosh et al. 2023).ApoE Isoforms Inhibit Amyloid Aggregation of Proinflammatory Protein S100A9.MDPI, Volume 25,Issue 410.3390/ijms25042114
Lacour et al. (2019). Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis. Polar Biol (2019). https://doi.org/10.1007/s00300-019-02507-2. - Protocols
- Agrisera Western Blot protocol and video tutorials
- Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
AS09 608 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)