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Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated, min, cross-reactivity to bovine,human, mouse IgG/serum
AS10 1146 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
- Product Info
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Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified IgG, F(ab)'2 fragment. Format: Lyophilized Quantity: 0.5 mg Reconstitution: For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Recommended dilution: 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB) - Additional Information
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Additional information: This antibody reacts with:heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins serum proteins from bovine, human or mouse IgG from human or mouse.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, Protease/IgG free and 0.1% (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
- Background
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Background: Goat anti-rabbit IgG (H&L), HRP conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to rabbit IgGs in immunological assays. Antibodies are adsorbed against bovine,human,mouse IgG/serum and affinity purified. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG. - Product Citations
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Selected references: Banday and Lajon (2017). Elevated systemic glutamic acid level in the non-obese diabetic mouse is Idd linked and induces beta cell apoptosis. Immunology. 2017 Feb;150(2):162-171. doi: 10.1111/imm - Protocols
- Antibody protocols
- Reviews:
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Viqar Banday | 2020-01-30This antibody works well for western blotting assay. I detected multiple antigens in the blot ranging from 36kDa to 66kDa. The result looks great with this antibody (1:10,000 dilution).
Accessories
AS10 1230 | Clonality: Polyclonal | Host:Goat | Reactivity: Rabbit IgG
AS10 1196 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS10 1233 | Clonality: Polyclonal | Host:Goat | Reactivity: Rabbit IgG (H&L)
AS10 1086 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)