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Goat anti-Rabbit IgG (H&L), DyLight® 800 conjugated, min, cross-reactivity to bovine, goat, human, mouse, rat IgG or serum proteins
AS12 2460 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
- Product Info
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Immunogen: Purified Rabbit IgG, whole molecule
Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Recommended dilution: 1 : 20-1 : 2000 for most applications - Application Examples
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Application example
5 µl of 15μg/µl Solanum lycopersicum protein saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody, fluorescent antibody (AS12 2460, Agrisera) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.
Courtesy of Dr. Betty Chung and Dr Zhengming Wang, University of Cambridge, United Kingdom - Additional Information
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Additional information: Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from bovine, goat, human, mouse or rat, IgG from bovine, goat, human, mouse or rat
- Background
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Background: Goat anti-rabbit IgG (H&L), DyLight®800 Conjugated, min. cross-reactivity to bovine, goat, human, mouse, rat IgG or serum proteins is a secondary antibody conjugated to DyLight® 800, which binds to Rabbit IgG (H&L) in immunological assays.
DyLight® 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)
DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
- Protocols
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