Goat anti-Rabbit IgG (H&L), ALP conjugated, min, cross-reactivity to bovine,goat,human, mouse,rat IgG

Product no: AS10 1086

AS10 1086  | Clonality: Polyclonal  |  Host: Goat  |  Reactivity: Rabbit IgG (H&L)

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Delivery:  3-6 business days
  • Product Info
  • Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgG.
    Format: Liquid
    Quantity: 1 mg
    Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Recommended dilution: 1 : 500 -1 : 2000 (ELISA), 1 : 50 -1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500 -1 : 2000 (WB)
  • Additional Information
  • Additional information:

    This antibody reacts with: heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

    Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins  IgG from bovine, goat, human, mouse or rat. 

    Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

  • Background
  • Background: Goat anti-rabbit IgG (H&L) is an ALP (alkaline phosphatase) conjugated secondary antibody which binds all rabbit IgGs in immunological assays. Antibody is adsorbed againstbovine,goat,human,mouse,rat IgG and affinity purified using solid phase rabbit IgG.
  • Product Citations
  • Selected references: Wężowicz et al. (2017). Interactions of arbuscular mycorrhizal and endophytic fungi improve seedling survival and growth in post-mining waste. Mycorrhiza. 2017 Mar 20. doi: 10.1007/s00572-017-0768-x.
  • Protocols
  • Antibody protocols
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