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Goat anti-Rabbit IgG Fc, HRP conjugated
AS10 858 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG Fc (two heavy chains with constant domains)
- Product Info
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Immunogen: Purified Rabbit IgG
Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: The optimal working dilution should be determined by the investigator - Reactivity
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Confirmed reactivity: Rabbit IgG Fc (two heavy chains with constant domains) Predicted reactivity: Rabbit IgG Fc (two Heavy chains with constant domains) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Additional information (application): This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis.
No reactivity is observed to the light chains on rabbit immunoglobulins or non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
This antibody will cross react with the heavy chain on other species IgG, but not with IgM or IgA.
- Background
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Background: Goat anti-rabbit IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to rabbit IgG Fc (two heavy chains with constant domains) in immunological assays.
- Protocols
- Antibody protocols
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Accessories
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