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Goat anti-Mouse IgG (H&L), HRP conjugated, min, cross-reactivity to bovine, goat,human, rabbit, rat IgG
AS10 964 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG (H&L)
- Product Info
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Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Recommended dilution: The optimal working dilution should be determined by the investigator, Suggested starting dilution: 1 : 20-1 : 2000 depending upon the application (immunolocalization or western blot) - Additional Information
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Additional information: This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins as well as bovine, goat, human, rabbit or rat IgG.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
- Background
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Background: Goat anti-mouse IgG (H&L) is a secondary antibody HRP conjugated which binds to all mouse IgGs in immunological assays. It is specially adsorbed against bovine, goat, human, rabbit and rat IgG. - Protocols
- Antibody protocols
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