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Goat anti-Human kappa chain, HRP conjugated, min, cross-reactivity to Mouse serum
- Product Info
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Immunogen: Purified human Kappa Chain BAA37169.1 Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Lyophilized Quantity: 0.5 mg Reconstitution: For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Recommended dilution: This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator - Additional Information
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Additional information: Purity of this preparation is >95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 1% (w/v) B, Protease/IgG free. Contains 0.1% (v/v) Kathon CG as preservative of bacterial growth.
Based on immunoelectrophoresis, this antibody reacts with: kappa light chains on human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to:non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins,mouse serum proteins.
- Background
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Background: Goat anti-human kappa (k) chain is a secondary antibody conjugated to HRP, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain. - Product Citations
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Selected references: Naderi et al. (2018). The Augmenting Effects of the tDNA Insulator on Stable Expression of Monoclonal Antibody in Chinese Hamster Ovary Cells. Monoclon Antib Immunodiagn Immunother. 2018 Nov;37(5):200-206. doi: 10.1089/mab.2018.0015. - Protocols
- Antibody protocols
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Accessories
- 10ml trial size is restricted to one unit per customer -
AS15 HRPstab | Bovine and mammalian protein free HRP and ALP-conjugate and protein stabilizer
This product can be purchased in 2 different volumes:
AS15 HRPstab-10, 10 ml. Trial size limited to one per customer
AS15 HRPstab-100, 100 ml
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