Goat anti-Human kappa chain, Biotin conjugated
- Product Info
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Immunogen: Purified human Kappa (k) Chain BAA37169.1 Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Liquid Quantity: 0.5 mg Storage: Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard. Recommended dilution: This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500 - 1 : 5 000 (IHC) - Additional Information
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Additional information: Purity of this preparation is >95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 1% (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.
- Background
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Background: Goat anti-human kappa (k) chain is a secondary antibody conjugated to biotin, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain. - Product Citations
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Selected references: Reinhart et al. (2014). In search of expression bottlenecks in recombinant CHO cell lines-a case study. Appl Microbiol Biotechnol. 2014 Feb 21. - Protocols
- Antibody protocols
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Accessories
AS10 1406 | Clonality: Polyclonal; | Host: Goat | Reactivity: Human kappa chain