Goat anti-Human IgM (” chain), F(ab)'2 fragment, ALP conjugated

Product no: AS10 1237

AS10 1237   | Clonality: Polyclonal  |  Host:Goat   |  Reactivity: Human IgM (µ chain)

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  • Product Info
  • Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgG, F(ab)'2 fragment.
    Format: Liquid
    Quantity: 0.5 mg
    Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Recommended dilution: 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)
  • Additional Information
  • Additional information:

    This antibody reacts with:heavy (µ) chains on human IgM based on immunoelectrophoresis.

    Minimum cross-reactivity is observed to: non-immunoglobulin serum proteins human IgG.

    Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1% (w/v) BSA, Protease/IgG free, 0.05% (w/v) sodium azide as preservative.

    Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)

  • Background
  • Background: Goat anti-human IgM (µ chain), ALP (alkaline phosphatase) conjugated is a F(ab)'2 fragment of a secondary antibody which binds to human IgM in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
  • Protocols
  • Antibody protocols
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