Goat anti-Chicken IgY (H&L), DyLight® 405 conjugated

Product no: AS16 3264
AS16 3264 | Clonality: Polyclonal | Host: Goat | Reactivity: Chicken IgY (H&L)
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  • Product Info
  • Immunogen: Purified Chicken IgY, whole molecule
    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
    Storage: Store freeze-dried powder at 2-8 °C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    Tested applications: Flow cytometry (Flow cyt), Immunolocalization (IL)
    Recommended dilution: 1 : 20-1 : 2000 (Flow cyt), (IL)
  • Reactivity
  • Confirmed reactivity: Chicken IgY
  • Additional Information
  • Additional information:

    Antibody purity is > 95% based on SDS-PAGE.
    DyLight® 405 (Ex = 400 nm; Em = 420 nm)
    Affinity purified using solid phase Chicken IgG/Y.
    Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.

    Based on IEP, this antibody reacts with: • heavy (γ) chains on chicken IgG/Y • light chains on all chicken immunoglobulins .
    Based on IEP, no reactivity is observed to: • non-immunoglobulin chicken serum immunoglobulins .

  • Background
  • Background: Goat anti-Chicken IgY (H&L) is a secondary antibody, conjugated to DyLight® 405, which binds to chicken IgY (H&L) in immunological assays.

    DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
  • Product Citations
  • Selected references: Huiming et al. (2020). Integration of Nodal and BMP Signaling by Mutual Signaling Effector Antagonism. Cell Rep. 2020 Apr 7;31(1):107487.doi: 10.1016/j.celrep.2020.03.051.
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