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Goat anti-Chicken IgY (H&L), ALP conjugated
AS09 606 | Clonality: Polyclonal | Host: Goat | Reactivity: Chicken IgY (H&L)
- Product Info
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Immunogen: purified chicken IgY, whole molecule
Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Liquid Quantity: 1 mg Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1 : 10 000 -1 : 100 000 (ELISA), 1 : 500 -1 : 2000 (IHC), 1 : 10 000-100 000 (WB) - Reactivity
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Confirmed reactivity: Chicken IgY heavy and light chains (H&L) Predicted reactivity: Chicken IgY Heavy and Light chains (H&L) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)
Antibody concentration is 1.14 mg/ml and it has been afinity purified on solid phase hen IgY (H&L)
Antibody is affinity purified using solid phase Chicken IgG/Y.AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
Additional information (application): No reactivity is observed to non-immunoglobulin hen serum proteins based in immunoelectrophoresis
chicken IgY is also called chicken or hen IgG
- Background
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Background: Goat anti-chicken IgY is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to hen IgY immunoglobulin in immunological assays.
- Protocols
- Agrisera Western Blot Guide
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Accessories
AS09 603 | Clonality: Polyclonal | Host: Goat | Reactivity: Hen IgY (H&L)
AS11 1822 | Clonality: Polyclonal | Host: Goat | Reactivity: Chicken IgY (H&L)
AS11 1823 | Clonality: Polyclonal | Host: Goat | Reactivity: Chicken IgY (H&L)