Anti-Glucuronoxylan (monoclonal, clone LM28)
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
- Product Info
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Sub class: IgM Immunogen: Polysaccharide Glucuronoxylan Host: Rat Clonality: Monoclonal Purity: Cell culture supernatant. Format: Liquid Quantity: 1 ml Storage: Store at +4°C (short term) and at -20°C (long term). Tested applications: ELISA (ELISA), Immuniflourescence (IF) Recommended dilution: 1:10 (ELISA, IF) - Reactivity
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Confirmed reactivity: Higher plants, ferns and mosses Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: Contains 0.05% Sodium Azide.
This antibody is made using a complex pectic immunogen.
It can recognise glucuronosyl substituted xylans and MeGlcA is not required for recognition. - Background
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Background: The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Glucuronoxylans are hemicellulosic plant cell wall polysaccharides which contains glucuronic acid and xylose. - Product Citations
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Selected references: Cornault et al. (2015). Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls. Planta. 2015 ec;242(6):1321-34.doi: 10.1007/s00425-015-2375-4.
- Protocols
- Immunofluorsecence as described in: Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.
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Accessories
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
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