Anti-GID1c | Gibberellin receptor GID1C

Name: Anti-GID1c | Gibberellin receptor GID1C
SKU: AS14 2800
Price: 319 EUR
Availability: InStock
Rating: 2 (1 reviews)

Product no: AS14 2800

AS14 2800 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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DATA SHEET IN PDF

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Product Info

Immunogen:	recombinant full-length Arabidopsis thaliana GID1c, UniProt: Q940G6 TAIR: At5g27320
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 �l
Reconstitution:	For reconstitution add 50 �l of sterile water
Storage:	Store lyophilized/reconstituted at -20�C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 5000 (WB)
Expected \| apparent MW:	38 kDa (Arabidopsis)

Reactivity

Confirmed reactivity:	Arabidopsis thaliana
Predicted reactivity:	Arabidopsis alpina, Ricinus communis Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Application Examples

application example

western blot using anti-GID1c antibodies

20 µg of total protein from Arabidopsis thaliana cauline leaves (C.L.), seedling (S.L.) material or imbibed seeds (I.S.) was extracted in 50 mM Phosphate buffer (pH 7.0) with 1X protease inhibitor (Sigma Aldrich) and was separated on a TGX and KD SDS-PAGE gel (BioRad) and blotted 14 min to PVDF using BioRad semi-dry turbo transfer system. Blots were blocked with 2% ECL advance (GE healthcare) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:75,000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with high sensitivity chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was 30 seconds.

Cauline leaves were collected after bolting but before anthesis, around 4 weeks of age (estimate).

Courtesy of Dr. Amber Hauvermale, Department of Crop and Soil Sciences, Washington State University, USA

Additional Information
Additional information (application): This antibody is also recognizng GST-GID1,
Background

Background:

GID1c (Gibberellin receptor GID1C) is a gibberellin (GA) receptor ortholog of the rice GA receptor OsGID1 which binds to GA and shows affinity to GA4 and interacts with DELLA proteins in vivo in the presence of GA4.

Alternative names: AtCXE19, Carboxylesterase 19, GID1-like protein 3, Protein GA INSENSITIVE DWARF 1C.

Product Citations

Selected references:

Hauvermale et al. (2015). Loss of Arabidopsis thaliana seed dormancy is associated with increased accumulation of the GID1 GA hormone receptors. Plant Cell Physiol. 2015 Jul 1. pii: pcv084.

Protocols
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Number of reviews: (1)

Hilla Schayek | 2016-11-07

0 | 0

I've used the antibody a few times according to the protocol and only one time it worked for me. 20 and 30 microgram protein gave a weak signal after ECL. but also a constant non-specific bend appeared.