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Anti-FtsZ2 | Plant cell division protein ftsZ2
AS13 2651 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
Benefits of using this antibody
- Product Info
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Immunogen: Recombinant part of Arabidopsis thalina FtsZ2 conserved in FtsZ2-1 UniProt: O82533, TAIR: At2g36250, FtsZ2-2 UniProt: Q9LXJ0, TAIR: AT3G52750
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 2000 (WB) Expected | apparent MW: 50.7 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare Predicted reactivity: Glycne max, Jatropha manihot, Ricinus communis
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example 
2 µg of recombinant FtsZ2 fragment(~10 kDa) (1) and total protein extracted with PEB AS08 300 from leaf tissue Arabidopsis thaliana (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with FtsZ2 antibody (AS13 2651, 1:2000, 1h) and secondary anti-rabbit (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602 in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. The signal was developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 2 min.
- Additional Information
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Additional information: Antibody available in 3 various pack sizes: 50, 100 and 150 µl - please, inquire - Background
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Background: FtsZ2 belongs to FtsZ family and binds to FtsZ1 and exhibits GTPase activity. Component of a plastid division machinery.
Alternative names: Plastid division protein FTSZ2-1, AtFtsZ2-1, Plastid division protein FTSZ2-2, AtFtsZ2-2. - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 413 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thalian, Hordeum vulgare
Benefits of using this antibody
AS10 715 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Candidatus Moranella endobia PCIT , Escherichia DH5a, Escherichia coli BW 25113, Shigella flexneri