Anti-FIE | Fertilization-independent endosperm

Name: Anti-FIE | Fertilization-independent endosperm
SKU: AS12 2616
Price: 237 EUR
Availability: InStock

Product no: AS12 2616

AS12 2616 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

237 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Data sheet
Product Info

Immunogen:	KLH-conjugated synthetic peptide derived from Arabidopsis thaliana FIE protein sequence, UniProt: Q9LT47, TAIR: At3g20740
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 �g
Reconstitution:	For reconstitution add 50 �l of sterile water
Storage:	Store lyophilized/reconstituted at -20�C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 1000 (WB)
Expected \| apparent MW:	41 kDa

Reactivity

Confirmed reactivity:	Recombinant FIE protein
Predicted reactivity:	Arabidopsis thaliana, Solanum lycopersicum, Triticum aestivum Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Additional Information

Additional information (application):

This antibody so far has been confirmed to detect recombinant FIE protein, GFP-tagged.

Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous FIE.

Background

Background:

FIE (fertilization-independent endosperm) belongs to polycomb group (PcG) protein which act by forming multiprotein complexes, required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.

Protocols
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