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Anti-Fer2 | Ferrritin 2
AS06 196 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Fer2 protein sequence (A8WCT5) from Chlamydomonas reinhardtii
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 200 µl Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 32 (with transitpeptide) | 16 (monomer) and ~60 (trimer) for Chlamydomonas reinhardtii
- Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii Predicted reactivity: Chlamydomonas reinhardtii
Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information (application): A picture with a western blot application example can be found in the reference given below - Background
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Background: Ferritin is the major iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In Chlamydomonas reinhardtii 2 isoforms, ferritin1 (Fer1) and ferritin2 (Fer2) are present. Both are plastid localized but Fer1 is quantitatively recovered in soluble extracts of cells while Fer2 is found in the particulate fraction. Partial purification of the Fer1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of Fer1 to Fer2 in iron-replete cells is 70:1 and indicates a more dominant role of Fer1 in iron homeostasis.
- Product Citations
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Selected references: Long et al. (2008) Genetics 179: 137-147
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Accessories
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