Donkey normal serum (10 ml)
Product no: AS10 1564
204
€
Delivery:
3-6 business days
- Product Info
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Host: Donkey Purity: Serum Format: Lyophilized Quantity: 10 ml Reconstitution: For reconstitution add 11.0 ml of sterile water Storage: Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 ºC. For storage at 2-8°C, add a preservative to prevent growth of bacteria. Rehydrate with 11.0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates. Tested applications: ELISA (ELISA). Immunofluorescence (IF), Western blot (WB) - Additional Information
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Additional information: Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.
For a Western Blot 10-20 ml of blocking agent is needed, aliquote and freeze the rest of the unused blocking buffer, because there are no preservatives in the serum.
Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.
As a blocking agent, this product is used to block non specific interaction with conjugates of donkey primary or secondary antibodies. - Background
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Background: Normal donkey serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications. - Protocols
- Antibody protocols
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